Amelioration of the adverse effects of thiram by 24- epibrassinolide in tomato (Solanum lycopersicum Mill.)

This study investigated thiram fungicide-induced-stress effects in tomato plants and the possible protective role of 24-epibrassinolide (24-EBL) response to (tetramethyl thiuram disulfide) toxicity. Tomato seedlings pretreated with 0, 10-11, 10-9 10-7 M 24-EBL were treated 6.6 mM thiram. leaves harvested 5 11 days after treatment (DAT) used for analysis. Thiram application caused oxidative stress by increased hydrogen peroxide malondialdehyde levels, whereas chlorophyll a, b carotenoid amounts total protein content decreased. In addition, activities antioxidant enzymes such as catalase, ascorbate peroxidase glutathione reductase decreased thiram-treated on DAT while pesticide detoxification (peroxidase glutathione- S-transferase) increased. The thiram-induced was alleviated pretreatments different concentrations 24-EBL. levels photosynthetic pigments pretreatments. further concentration under stress, most effective determined 10-11 results suggested that could effectively alleviate phytotoxicity plants.